84-5501-71　［取扱停止］JNK1/2/3, phosphorylated (Thr183) 100ul　538167

特徴

• Mitogen-activated protein kinase 8 encoded by MAPK8 is a member of the MAP kinase family.
• MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.
• Mitogen-activated protein kinase 8 is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli.
• The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis.
• Mitogen-activated protein kinase 8 is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway.
• Studies of the mouse counterpart of MAPK8 suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.
• Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.