MAPK8IP1 (mitogen-activated protein kinase 8 interacting protein 1) encodes a regulator of the pancreatic beta-cell function.
It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun N-terminal kinase (Mapk8).
This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells.
This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2.
RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells.
MAPK8IP1 is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human JIP-1 only when phosphorylated at T103. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human JIP-1 around the phosphorylation site of T103.
