JAK2 is a kinase that is misregulated or mutated in a number of myeloproliferative diseases and cancers.
The mutation V617F is the most clinically relevant variant, and is seen in around half of myeloproliferative disorders.
The variant is a known activating mutation, and activated JAK2 is sufficient to drive myeloproliferative disorders in mouse models.
V617F, while most recurrent, is not the only mechanism by which JAK2 can be activated in patients.
JAK2 is now one of the first diagnostic markers tested upon diagnosis with a myeloproliferative disorder.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunocytochemistry: 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of JAK2/3 when Phospho occurs at Tyr966/939(human) or Tyr966/935(mouse/rat), 538146 doesn't react with total protein. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human JAK2/3 (Phospho-Tyr966/939) at amino acid sequence of T966.
