IL12B encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities.
Interleukin 12 is a disulfide-linked heterodimer composed of the 40kD cytokine receptor like subunit encoded by IL12B, and a 35kD subunit encoded by IL12A.
This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development.
This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen.
Overexpression of IL12B was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.
The promoter polymorphism of IL12B has been reported to be associated with the severity of atopic and non-atopic asthma in children.
Applications:Suitable for use in Western Blot, Immunohistochemistry (paraffin), ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:500-1:200ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human IL-12B p40.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa241-290 from human IL-12B p40.
