Interleukin-10 encoded by IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes.
This cytokine has pleiotropic effects in immunoregulation and inflammation.
It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages.
It also enhances B cell survival, proliferation, and antibody production.
This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway.
Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human IL-10.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the Internal region of human IL-10.
