NFKBIA encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains.
NFKB inhibitor alpha interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses.
NFKB inhibitor alpha moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export.
Mutations in NFKBIA have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human IκB-α protein only when phosphorylated at S32/S36. Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide derived from human IkappaB-alpha around the phosphorylation site of S32/S36
