HM74, also known as PUMAG or Puma-g, is a member of the G protein coupled receptor (GPCR) superfamily.
In humans, HM74 is encoded by two different genes (GPR109A and GPR109B) that express two distinct proteins, namely HM74A and HM74B (also known as simply HM74), which are 96% homologous.
In mice and rats, only one gene (deisngnated Gpr109a) encodes the HM74 protein.
HM74B is a Gi protein-coupled receptor that mediates the metabolic effects of nicotinic acid.
Localizing to the cell membrane, HM74B is highly expressed in adipocytes, immune cells and spleen and, like all members of the GPCR superfamily, contains seven transmembrane domains.
HM74B lacks the characteristic N-linked glycosylation sites that are present in other GPCR family members and also shows a more diverged amino acid sequence homology from most family members, implying different ligand specificity.
Applications:Suitable for use in Western Blot, Immunofluorescence and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human HM74. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa270-350 from the C-terminal region of human HM74.
