HLA-A (major histocompatibility complex, class I, A) belongs to the HLA class I heavy chain paralogues.
This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin).
The heavy chain is anchored in the membrane.
Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen.
They are expressed in nearly all cells.
The heavy chain is approximately 45kD and its gene contains 8 exons.
Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule.
Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
Hundreds of HLA-A alleles have been described.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of HLA Class I. Species Crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to HLA Class I.
