Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4).
The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
HIST1H3B is intronless and encodes a replication-dependent histone that is a member of the histone H3 family.
Transcripts from HIST1H3B lack polyA tails; instead, they contain a palindromic termination element.
HIST1H3B is found in the large histone gene cluster on chromosome 6p22-p21.3.
Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunocytochemistry: 1:100Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Histone H3 (Tri-Methyl-K5), while it doesn't react with total or Di-Methy or mono-Methyl. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to K5aa from the internal region of human Histone H3 (Tri-Methyl-K5).
