A Polyhistidine-Tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N-terminal or C-terminal of the protein.
It is also known as hexa histidine-tag, 6xHis-Tag, and by the trademarked name His-Tag.
Polyhistidine-Tags are often used for affinity purification of Polyhistidine-Tag recombinant proteins expressed in E.coli and other prokaryotic expression systems.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 50% glycerol. Lableled with horseradish peroxidase (HRP).
Specificity：Recognizes His-Tag.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：Synthetic peptide corresponding to His-Tag corresponding to E.coli.
