The His-Tag is a series of six to nine histidine residues generally fused to either the C-terminal or N-terminal of a recombinant protein.
The small size of the His-Tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions.
The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper.
The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes His-Tag recombinant proteins or His-Tag proteins overexpressed in cells.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Synthetic peptide corresponding to His-Tag, conjugated to KLH.
