A Polyhistidine-Tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N-terminal or C-terminal of the protein.
It is also known as hexa Histidine-Tag, 6xHis-Tag, and by the trademarked name His-Tag.
Polyhistidine-Tags are often used for affinity purification of Polyhistidine-Tag recombinant proteins expressed in E.coli and other prokaryotic expression systems.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes His-Tag.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：Synthetic peptide corresponding to His-Tag corresponding to E.coli.
