HIRA (histone cell cycle regulator) encodes a histone chaperone that preferentially places the variant histone H3.
in nucleosomes.
Orthologs of HIRA in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin.
This gene plays an important role in the formation of the senescence-associated heterochromatin foci.
These foci likely mediate the irreversible cell cycle changes that occur in senescent cells.
It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human HIRA (phospho Thr555). Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human HIRA around the phosphorylation site of T555.
