GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum.
GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest.
Antibodies to GST are useful for checking protein expression both in plaques and on Western Blot as well as for immunoaffinity purification of proteins expressed in cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes C-terminal, internal, and N-terminal GST fusion proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：Recombinant protein corresponding to GST-Tag.
