84-5479-71　［取扱停止］GPX3 30ul　536962

特徴

• Glutathione peroxidase 3 encoded by GPX3 belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage.
• Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity.
• This isozyme is secreted, and is abundantly found in plasma.
• Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia.
• This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site.
• Sec is encoded by the UGA codon, which normally signals translation termination.
• The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal.
• Alternatively spliced transcript variants have been found for this gene.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.