G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways.
Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK.
Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems.
Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner.
Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation.
Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells.
Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation.
Stimulates uterine epithelial cell proliferation.
Enhances uterine contractility in response to oxytocin.
Contributes to thymic atrophy by inducing apoptosis.
Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules.
Promotes neuritogenesis in developing hippocampal neurons.
Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death.
Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons.
Inhibits early osteoblast proliferation at growth plate during skeletal development.
Inhibits mature adipocyte differentiation and lipid accumulation.
Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells.
Functions also as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway.
Involved in cancer progression regulation.
Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway.
Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1).
Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells.
Applications:Suitable for use in Western Blot, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human GPR144.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa60-140 of human GPR144 at N-terminal.
