The green fluorescent protein (GFP) is a protein composed of 238aa residues (26.9kD) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.
Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish.
The GFP has a major excitation peak at a wavelength of 395nm and a minor one at 475nm.
Its emission peak is at 509nm, which is in the lower green portion of the visible spectrum.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 50% glycerol. Lableled with horseradish peroxidase (HRP).
Specificity：Recognizes GFP-Tag at ~26.9kD.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：Recombinant protein corresponding to GFP-Tag.
