TUBGCP4 encodes a component of the gamma-tubulin ring complex, gamma-tubulin complex component 4, which is required for microtubule nucleation.
In mammalian cells, the protein localizes to centrosomes in association with gamma-tubulin.
Crystal structure analysis revealed a structure composed of five helical bundles arranged around conserved hydrophobic cores.
An exposed surface area located in the C-terminal domain is essential and sufficient for direct binding to gamma-tubulin.
Mutations in this gene that alter microtubule organization are associated with microcephaly and chorioretinopathy.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human GCP4. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa160-240 of human GCP4.
