Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.
These subunits are encoded by families of related genes.
GNB3 encodes a beta subunit which belongs to the WD repeat G protein beta family.
Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.
A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity.
This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity.
GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites.
Alternative splicing results in multiple transcript variants.
Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human GBB3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human GBB3 protein.
