84-5472-95　［取扱停止］GAPDH 200ul　536608

特徴

• GAPDH encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family.
• Glyceraldehyde-3-phosphate dehydrogenase has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions.
• The product of GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD).
• Glyceraldehyde-3-phosphate dehydrogenase has additionally been identified to have uracil DNA glycosylase activity in the nucleus.
• Also, Glyceraldehyde-3-phosphate dehydrogenase contains a peptide that has antimicrobial activity against E.
• coli, P.
• aeruginosa, and C.
• albicans.
• Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage.
• Many pseudogenes similar to this locus are present in the human genome.
• Alternative splicing results in multiple transcript variants.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:5000Immunohistochemistry (paraffin): 1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.