84-5472-45　GAL4 DNA Binding Domain 100ul　536569

特徴

• The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators.
• Since the N-terminal 147aa residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation , this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins.
• This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site.
• Fusions between GAL4 (aa1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells.
• A unique "two-hybrid" system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:10,000-1:50,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.