GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16kD; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport.
Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy.
When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: gABARAPL2-I, later conjugated to PE lipid as lipidated form: gABARAPL2-II.
The lipidated form migrates faster than its mature form on SDS-PAGE gel.
GABARAPL2 is strongly expressed in the brain.
This antibody is specific to GABARAPL2.
It has no cross-reaction to other GABARAP protein.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of GABARAP. Species Crossreactivity: human, mouse, and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to GABARAP.
