FXYD5 encodes a member of a family of small membrane proteins that share a 35aa signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids.
The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator.
Mouse FXYD5 has been termed RIC (Related to Ion Channel).
FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme.
FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems.
Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminal extracellular and the C-terminal on the cytoplasmic side of the membrane.
This gene product, FXYD5, is a glycoprotein that functions in the up-regulation of chemokine production, and it is involved in the reduction of cell adhesion via its ability to down-regulate E-cadherin.
It also promotes metastasis, and has been linked to a variety of cancers.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human FXYD5.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human FXYD5 at aa sequence of Internal.
