Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways.
FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue.
FRS2 is a 508 amino-acid protein, which is phosphorylated on tyrosine residues.
The molecular weight of non-phosphorylated FRS2 is 57-68kD, but phosphorylated FRS2 is 80-90kD.
Phosphorylation of FRS2 is associated with activation of a number of MAP kinases.
Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human FRS2 (phospho Tyr436). Species Crossreactivity: mouse and monkey
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Synthetic peptide corresponding to human FRS2 around the phosphorylation site of Y436.
