Zinc finger CCCH domain-containing protein 8 acts as a transcriptional repressor of the GATA3 promoter.
Sequence-specific DNA-binding factor that binds to the 5-AGGTCTC-3 sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity).
Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:23932780).
Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.
Among its related pathways are gene expression and RNA polymerase II transcription initiation and promoter clearance.
GO annotations related to this gene include poly (A) RNA binding and sequence-specific DNA binding.
An important paralog of this gene is ZC3H4.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Fliz1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa50-130 of human Fliz1.
