The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors.
This peptide can be expressed and detected with the protein of interest as an N-terminal or C-terminal fusion.
N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag.
The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature.
As a result, the FLAG peptide is more likely to be accessible to antibodies.
A FLAG-Tag can be used in many different assays that require recognition by an antibody, such as western blot, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes FLAG-Tag recombinant proteins or FLAG-Tag proteins overexpressed in cells.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Synthetic peptide corresponding to FLAG-Tag, conjugated to KLH.
