FAT10, also designated ubiquitin D or diubiquitin, is a 165aa protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin.
Each domain contains two cysteines, along with a free C-terminal diglycine motif required for FAT10 conjugate formation.
FAT10 is inducible by interferon-g and tumor necrosis factor a (TNF).
The FAT10 protein interacts with MAD2, a component of the spindle checkpoint, and plays a role in antigen presentation, cytokine response, apoptosis and mitosis.
It may also regulate cell growth during dendritic cell or B cell activation and development.
FAT10 mRNA is expressed mainly in some dendritic cells and lymphoblastoid lines and in other specific cells subsequent to interferon-g induction.
The human FAT10 gene, designated UBD, maps to chromosome 6p21.
and is overexpressed in the tumors of various epithelial cancers.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human FAT10.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the Internal region of human FAT10.
