84-5464-93　［取扱停止］Factor XIIIa, cleaved (G39) 30ul　536191

特徴

• F13A1 encodes the coagulation factor XIII A subunit.
• Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade.
• Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits.
• The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules.
• Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin.
• Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII.
• This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot.
• It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
• Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone.
• These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.