MAPK15 (mitogen-activated protein kinase 15, ERK8) is a 544aa protein that belongs to the CMGC Ser/Thr protein kinase family (MAP kinase subfamily).
MAP kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis.
MAPK15 functions as a catalytic kinase using ATP to produce ADP and a phosphoprotein.
A TXY motif, containing one threonine and one tyrosine residue, activates the MAP kinases upon phosphorylation.
MAPK15 is a ubiquitously expressed protein with highest expression found in lung and kidney.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human ERK 8. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human ERK 8 around the non-phosphorylation site of T175/Y177.
