84-5461-19　［取扱停止］Epac 30ul　535992

特徴

• RAPGEF3 (Rap Guanine Nucleotide Exchange Factor 3) is a Protein Coding gene.
• Among its related pathways are Platelet activation, signaling and aggregation and Signaling by GPCR.
• GO annotations related to this gene include guanyl-nucleotide exchange factor activity and cAMP-dependent protein kinase regulator activity.
• An important paralog of this gene is RASGEF1A.
• uanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP.
• Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis.
• Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling.
• Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.
• TPases are a group of enzymes that catalyze hydrolysis of the gamma phosphate bond in guanine triphosphate (GTP) to form guanine diphosphate (GDP).
• Mg2+ ions are essential for catalytic activity.
• GTPases, often coupled to G proteins, are essential in signal transduction.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.