Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin.
Humans have six elastase genes which encode structurally similar proteins.
Elastase, neutrophil expressed is proteolytically processed to generate the active protease.
Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix.
The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin.
Elastase, neutrophil expressed also degrades the outer membrane protein A (OmpA) of E.coli as well as the virulence factors of such bacteria as shigella, salmonella and yersinia.
Mutations in ELANE are associated with cyclic neutropenia and severe congenital neutropenia (SCN).
ELANE is present in a gene cluster on chromosome 19.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human ELNE. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human ELNE.
