ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily.
Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene.
The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade.
This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing.
Related pseudogenes have been identified on chromosomes 7 and 14.
Applications:Suitable for use in Immunohistochemistry, Immunoprecipitation, and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300Immunoprecipitation (lysate): 2-5ug/mgELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Elk-1 (phospho Thr417). Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC IP
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Elk-1 around the phosphorylation site of T417.
