84-5459-37　［取扱停止］eIF4G, phosphorylated (Ser1148) 30ul　535899

特徴

• The eukaryotic translation initiation factor 4 gamma 1 encoded by EIF4G1 is a component of the multi-subunit protein complex EIF4F.
• This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis.
• The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex.
• The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex.
• A domain at its N-terminal can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation.
• Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
• Applications:Suitable for use in Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.