MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a 63kD (predicted) novel transmembrane glycoprotein that shares some homology to beta ‑chains of laminin.
It is expressed by hepatocytes, cerebellar Purkinje cells, Schwann cells, keratinocytes and intestinal epithelium.
MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration.
Mature human MEGF9 is 572aa in length.
It is a single span type I transmembrane protein that contains a 484aa extracellular region (aa31‑514) plus a 68aa C‑terminal cytoplasmic domain.
The extracellular region possesses a lengthy Pro‑rich region (aa55‑200), followed by five EGF‑like domains (aa204‑451).
MEGF9 may run at approximately 160kD in SDS‑PAGE, suggesting either heavy glycosylation or dimerization.
Over aa36‑514, human MEGF9 shares 76% aa sequence identity with mouse MEGF9.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human EGFL5. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa360-440 of human EGFL5.
