EGF encodes a member of the epidermal growth factor superfamily.
The encoded preproprotein is proteolytically processed to generate the 53aa epidermal growth factor peptide.
This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types.
This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor.
Defects in this gene are the cause of hypomagnesemia type 4.
Dysregulation of this gene has been associated with the growth and progression of certain cancers.
Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human EGF. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the Internal region of human EGF.
