The downstream of kinase family (Dok-1-7) are members of a class of docking proteins that interact with receptor tyrosine kinases and, via this interaction, mediate biological responses within the body.
Dok-4 (Downstream of kinase-4) is a 326aa that contains one PH domain and one IRS-type PTB domain and belongs to the Dok family of interacting proteins.
Expressed in a variety of tissues with highest expression in liver, heart, kidney and skeletal muscle, Dok-4 plays an important role in Ret-mediated neurite outgrowth and may link Ret with downstream effectors during neuronal differentiation.
Additionally, Dok-4 is thought to play a positive role in the activation of MAPK pathways and may participate in T-cell induced immune system regulation.
Overexpression of Dok-4 is associated with clear cell renal cell carcinoma, suggesting a role for Dok-4 in tumorigenesis.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300Immunofluorescence: 1:200-1:1000ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Dok-4. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa30-110 of human Dok-4 at N-terminal.
