CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation.
Studies in mice have demonstrated that DNA methylation is required for mammalian development.
This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation.
The protein localizes primarily to the nucleus and its expression is developmentally regulated.
Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.
Eight alternatively spliced transcript variants have been described.
The full length sequences of variants 4 and 5 have not been determined.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Dnmt3b at ~96kD. Species Crossreactivity: chicken
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Ch Hu
Immunogen：Synthetic peptide corresponding to aa1-80 of human Dnmt3b at N-terminal.
