84-5453-85　［取扱停止］DNA pol Iota 30ul　535612

特徴

• POLI (Polymerase (DNA) Iota) is a Protein Coding gene.
• Among its related pathways are DNA Double-Strand Break Repair and Fanconi anemia pathway.
• GO annotations related to this gene include damaged DNA binding and DNA-directed DNA polymerase activity.
• An important paralog of this gene is POLH.
• rror-prone DNA polymerase specifically involved in DNA repair.
• Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.
• Favors Hoogsteen base-pairing in the active site.
• Inserts the correct base with high-fidelity opposite an adenosine template.
• Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.
• May play a role in hypermutation of immunogobulin genes.
• Forms a Schiff base with 5-deoxyribose phosphate at abasic sites, but may not have lyase activity.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.