DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases.
In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches.
These exonucleases include the family of DNA polymerases.
DNA pol alpha, beta, delta, and e are involved in DNA replication and repair.
DNA pol delta and DNA pol e are multisubunit enzymes, with DNA pol delta consisting of two subunits p125, which interacts with the sliding DNA clamp protein PCNA, and p50.
The nuclear-encoded DNA pol gamma is the only DNA polymerase required for the replication of the mitochondrial DNA.
DNA pol omega is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis.
DNA pol œ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.
Applications:Suitable for use in Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human DNA pol delta. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa200-280 of human DNA pol delta at N-terminal.
