Dystrophia myotonica protein kinase encoded by DMPK is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases.
Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman.
The 3' untranslated region of this gene contains 5-38 copies of a CTG trinucleotide repeat.
Expansion of this unstable motif to 50-5000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number.
Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region.
Several alternatively spliced transcript variants of DMPK have been described, but the full-length nature of some of these variants has not been determined.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human DMPK.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa30-110 of human DMPK at N-terminal.
