Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes.
Nine mammalian isotypes have been identified, which are encoded by separate genes.
Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD).
Diacylglycerol kinase beta encoded by DGKB is a diacylglycerol kinase, beta isotype.
Two alternatively spliced transcript variants have been found for this gene.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of human DGK-beta. Species Crossreactivity: mouse and rat
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to aa630-710 of human DGK-beta at C-terminal.