DENND5A (DENN domain containing 5A) encodes a DENN-domain-containing protein that functions as a RAB-activating guanine nucleotide exchange factor (GEF).
This protein catalyzes the conversion of GDP to GTP and thereby converts inactive GDP-bound Rab proteins into their active GTP-bound form.
The encoded protein is recruited by RAB6 onto golgi membranes and is therefore referred to as RAB6-interacting protein 1.
This protein binds with RAB39 as well.
Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Mutations in DENND5A are associated with early infantile epileptic encephalopathy-49.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human DEN5A. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa650-730 of human DEN5A.
