The protein encoded by DDX3Y (DEAD-box helicase 3, Y-linked) is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif.
These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions.
This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of DDX3Y is not complemented by DDX3X.
Mutations in DDX3Y result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome.
Pseudogenes sharing similarity to both DDX3Y and the DDX3X paralog are found on chromosome 4 and the X chromosome.
Alternative splicing results in multiple transcript variants encoding different isoforms.
Applications:Suitable for use in Immunohistochemistry, ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human DDX3Y. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding toaa10-90 from the region of human DDX3Y at N-terminal.
