84-5451-40　［取扱停止］DDX3 30ul　535488

特徴

• DEAD-box helicase 3, X-linkedencoded by DDX3X is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity.
• This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA.
• This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm.
• Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export.
• In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication.
• Misregulation of DDX3X has been implicated in tumorigenesis.
• This gene has a paralog located in the nonrecombining region of the Y chromosome.
• Pseudogenes sharing similarity to both DDX3X and the DDX3Y paralog are found on chromosome 4 and the X chromosome.
• Alternative splicing results in multiple transcript variants.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.