The DYKDDDDK peptide (Flag-Tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology.
It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism.
It can also be used in the isolation of protein complexes with multiple subunits.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes DDDDK-Tag.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：Synthetic peptide corresponding to DDDDK-Tag.
