The YTH domain family protein family (YTHDF) includes YTHDF1, YTHDF2 and TYHDF3.
YTHDF1, also designated Dermatomyositis associated with cancer putative autoantigen 1 (DACA-1), is a 559aa protein that contains one YTH domain, which is a potential RNA binding domain.
YTHDF2, also designated High-glucose-regulated protein 8, CLL-associated antigen KW-14 or Renal carcinoma antigen NY-REN-2, is a 579aa protein that also contains one YTH domain.
YTHDF3 is a 585aa protein that also contains one YTH domain.
The gene encoding YTHDF1 maps to chromosome 20, which houses over 600 genes, some of which are associated with Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome.
Additionally, chromosome 20 contains a region with numerous genes which are thought important for seminal production and may be potential targets for male contraception.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：30ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human DACA-1. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1-80 of human DACA-1 at N-terminal.
