KRT8 is a member of the type II keratin family clustered on the long arm of chromosome 12.
Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells.
The product of KRT8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells.
Keratin 8 plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation.
Mutations in KRT8 cause cryptogenic cirrhosis.
Alternatively spliced transcript variants have been found for KRT8.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Cytokeratin 8 protein only when acetylated at K483.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the human Cytokeratin 8 around the acetylation site of K483.
