84-5447-46　［取扱停止］Cyclin L1 30ul　535257

特徴

• Cell proliferation is controlled at specific stages of the cell cycle by distinct protein kinase complexes.
• These complexes consist of a catalytic subunit associating with a specific regulatory subunit to form the active kinase.
• The cyclins, which include cyclin A, B, C, D, E, F, G, H, I, K, L, T and their related proteins, including Dbf4, comprise the regulatory subunits of these kinase complexes.
• The controlled activation of the kinase complexes at various intervals of the cell cycle is regulated by the availability of the cyclins to the catalytic subunit.
• Unlike the catalytic subunit, which is expressed continually, the expression and stability of the regulatory subunit fluctuates depending on the stage of the cell cycle and, thereby, regulates the kinase activity.
• Cyclin L1 is a ubiquitously expressed nuclear protein that can be detected in higher levels in thymus.
• In neck and head squamous cell carcinomas, cyclin L1 can be overexpressed and is therefore often considered a proto-oncogene.
• It interacts with POLR2A, CDC2L and SFRS2.
• Cyclin L1 plays a role in the mRNA splicing process regulation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.