CYB561D2 (Cytochrome b561 domain-containing protein 2), also known as putative tumor suppressor protein 101F6, is a 222aa highly hydrophobic protein that contains six membrane spanning domains.
Localized to endosomes and endoplasmic reticulum of the perinuclear region, CYB561D2 contains 2 heme-binding sites, through which it reduces ferric ions.
CYB561D2 is ubiquitously expressed, with highest levels in lung, liver and kidney.
Though expression of CYB561D2 is abundant in normal lung bronchial epithileal cells, CYB561D2 is absent in most lung cancers.
The gene encoding CYB561D2 maps to human chromosome 3p21.3, a region that is prone to genetic alterations and is associated with frequent and early allele loss in many human cancers.
Exogenous expression of CYB561D2 leads to the induction of caspase-independent apoptotic and autophagic pathways, resulting in the synergistic killing of tumor cells.
This suggests an important role for CYB561D2 as a therapeutic intervention in cancer treatment.
Applications:Suitable for use in Western Blot, Immunofluorescence, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000ELISA: 1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human CYB561D1.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa160-240 of human CYB561D1 at C-terminal.
