84-5445-33　［取扱停止］Crystallin-alphaB, phosphorylated (Ser19) 100ul　535148

特徴

• Mammalian lens crystallins are divided into alpha, beta, and gamma families.
• Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively.
• Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family.
• They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates.
• Post-translational modifications decrease the ability to chaperone.
• These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively.
• Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture.
• The crystallin alpha B has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions.
• Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs.
• Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
• Alternative splicing results in multiple transcript variants.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.